Journal: Oncotarget
Article Title: Deregulated expression of NKL homeobox genes in T-cell lymphomas
doi: 10.18632/oncotarget.26929
Figure Lengend Snippet: ( A ) RQ-PCR analysis of BMP4 in selected cell lines and primary cells. Data for BMP4 protein levels in the supernatants as obtained by ELISA are indicated (insert). ( B ) RQ-PCR analysis of MSX1 in DERL-2 and DERL-7 cells treated with recombinant BMP4 (left) and with dorsomorphin (DM) (right). ( C ) RQ-PCR analysis for PDGFRB and BMP4 of DERL-2 cells (left) and DERL-7 cells (right) treated for siRNA-mediated knockdown of PDGFRB. ( D ) RQ-PCR analysis of BMP4 in DERL-2 (left) and DERL-7 (right) treated with 1 ng/ml or 10 ng/ml recombinant PDGFD. ( E ) RQ-PCR analysis for PDGFD and BMP4 of DERL-7 cells treated for siRNA-mediated knockdown of PDGFD.
Article Snippet: Cells were variously treated for 16 h with 20 ng/ml recombinant BMP4, with 1 or 10 ng/ml recombinant PDGFD (R & D Systems, Abingdon, UK), with 100 μM Dasatinib (LC Laboratories, Woburn, MA), with 5 μM BMP receptor inhibitor dorsomorphin (DM) (Calbiochem, Darmstadt, Germany) dissolved in dimethyl sulfoxide (DMSO), with 10 μM EZH2-inhibitor DZNep (Sigma, Taufkirchen, Germany), or with 10 μg/ml histone deacetylase inhibitor trichostatin A (TSA) (Sigma).
Techniques: Enzyme-linked Immunosorbent Assay, Recombinant